BACTERICIDAL ACTIVITY PHORBAZOLE E ON GROWTH STAPHYLOCOCCUS AUREUS
Active compounds from marine organisms for the treatment of various types of diseases have
been found, since marine organisms are a source of secondary metabolites and also a source of
organic compounds of natural materials. Phorbazoles basic framework can be synthesized from 1-
(p - tosyl groups) – pyrol- 2 - carbonyl chloride and phenacyl ammonium chloride salts .
Staphylococcus aureus is a gram-positive bacteria, pathogens, blood cells hemolysis, plasm
coagulate, and produce extracellular enzymes and toxins .
The aim of research to find out phorbazole E has bactericidal activity against the growth of S.
aureus and at concentrations how phorbazole E most capable of causing bactericidal activity
against S. aureus growth. The total number of 36 experimental units petri dishes and test tubes 36
(9 control units, 9 units of concentration of 10 mg/500 ml, 9 units of concentration of 18.75 mg/500
ml, 9 units of concentration of 37.5 mg/500 ml). The first test using Mueller Hinton broth at about 9
ml test tube is then inserted isolate suspension of 1 ml, incubation at a temperature of 370C for 24
hours to determine the bactericidal activity. The second test using Mueller Hinton agar in Petri
dishes to determine the inhibition zone .
Obtained from laboratory tests: phorbazole E has bactericidal activity against S. aureus growth.
Concentration of 37.5 mg/500 ml has bactericidal activity that is able to inhibit the growth of S.